Western blotting analysis using centrosomal protein 55 antibody (Cat#65058). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with centrosomal protein 55 antibody (Cat#65058, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using centrosomal protein 55 antibody (Cat#65058). Centrosomal protein 55 expression in wild-type (WT) and centrosomal protein 55 (CEP55) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with centrosomal protein 55 antibody (Cat#65058, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using centrosomal protein 55 antibody (Cat#65058, 1:1,000), Top panel: wild-type (WT); Bottom panal: CEP55 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Centrosomal protein 55 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using Centrosomal protein 55 antibody (Cat#65058, 1:1,000). Green, isotype control; red, Centrosomal protein 55.
