Western blotting analysis using N-acetylglucosamine kinase antibody (Cat#65080). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with N-acetylglucosamine kinase antibody (Cat#65080, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using N-acetylglucosamine kinase antibody (Cat#65080). N-acetylglucosamine kinase expression in wild-type (WT) and N-acetylglucosamine kinase (NAGK) knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with N-acetylglucosamine kinase antibody (Cat#65080, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of N-acetylglucosamine kinase expression in HepG2 cells using N-acetylglucosamine kinase antibody (Cat#65080, 1:2,000). Green, isotype control; red, N-acetylglucosamine kinase.
Immunocytochemical staining of HepG2 cells with N-acetylglucosamine kinase antibody (Cat#65080, 1:1,000) . Nuclei were stained blue with DAPI; N-acetylglucosamine kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using n-acetylglucosamine kinase antibody (Cat#65080, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
