Western blotting analysis using IMMT antibody (Cat#65109). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IMMT antibody (Cat#65109, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using IMMT antibody (Cat#65109). IMMT expression in wild-type (WT) and IMMT shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IMMT antibody (Cat#65109, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IMMT expression in HepG2 cells using IMMT antibody (Cat#65109, 1:1,000) . Green, isotype control; red, IMMT.
Immunocytochemical staining of HepG2 cells with IMMT antibody (Cat#65109, 1:1,000) . Nuclei were stained blue with DAPI; IMMT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human renal carcinoma using IMMT antibody (Cat#65109, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
