Western blotting analysis using RAC1 antibody (Cat#6519). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAC1 antibody (Cat#6519, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:4,000
Note
This product is for research use only.
Data
Western blotting analysis using RAC1 antibody (Cat#6519). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAC1 antibody (Cat#6519, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RAC1 antibody (Cat#6519). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAC1 antibody (Cat#6519, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001011s ]
SELECT * FROM `goods` WHERE `code` = '6519' LIMIT 1 [ RunTime:0.000607s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000615s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000477s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000548s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10335 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000394s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000413s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10335 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000263s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000363s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000290s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053894s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4506,9748,8339,9532,5260,8467,9056,9438,4687,310) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000866s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000526s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000323s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000738s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000351s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000458s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000495s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000268s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000213s ]