Western blotting analysis using MTHFR antibody (Cat#65213). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MTHFR antibody (Cat#65213). MTHFR expression in wild-type (WT) and MTHFR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MTHFR expression in HeLa cells using MTHFR antibody (Cat#65213, 1:2,000). Green, isotype control; red, MTHFR.
Validation of MTHFR knockdown using flow cytometry. Wild-type(WT, Blue) and MTHFR knockdown(KD, Green) HeLa cells were stained with MTHFR antibody(Cat#65213, 1:2,000) and analyzed using BD flow cytometer.
