Western blotting analysis using DYNLT1 antibody (Cat#65226). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DYNLT1 antibody (Cat#65226, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using DYNLT1 antibody (Cat#65226). DYNLT1 expression in wild-type (WT) and DYNLT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DYNLT1 antibody (Cat#65226, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using DYNLT1 antibody (Cat#65226, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HeLa cells using DYNLT1 antibody (Cat#65226, 1:1,000), Top panel: wild-type (WT); Bottom panal: DYNLT1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; DYNLT1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of DYNLT1 expression in HT-1080 cells using DYNLT1 antibody (Cat#65226, 1:2,000). Green, isotype control; red, DYNLT1.
Validation of DYNLT1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DYNLT1 antibody (Cat#65226, 1:2,000) and analyzed using BD flow cytometer.
