Western blotting analysis using adducin 1 antibody (Cat#65299). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with adducin 1 antibody (Cat#65299, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using adducin 1 antibody (Cat#65299). Adducin 1 expression in wild-type (WT) and adducin 1 (ADD1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with adducin 1 antibody (Cat#65299, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Adducin 1 expression in HT-1080 cells using Adducin 1 antibody (Cat#65299, 1:2,000). Green, isotype control; red, Adducin 1.
Validation of Adducin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with Adducin 1 antibody (Cat#65299, 1:2,000) and analyzed using BD flow cytometer.
Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using adducin 1 antibody (Cat#65299, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
