Western blotting analysis using annexin A1 antibody (Cat#6557). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A1 antibody (Cat#6557, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with annexin A1 antibody (Cat#6557, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human Annexin A1
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using annexin A1 antibody (Cat#6557). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A1 antibody (Cat#6557, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with annexin A1 antibody (Cat#6557, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using annexin A1 antibody (Cat#6557). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A1 antibody (Cat#6557, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with annexin A1 antibody (Cat#6557, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.005380s ]
SELECT * FROM `goods` WHERE `code` = '6557' LIMIT 1 [ RunTime:0.001502s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001364s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001421s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000458s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10301 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000513s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001512s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10301 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000737s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001027s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.002167s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.288710s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4893,8985,4440,93,10186,7231,9334,4470,4940,9390) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.006023s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000744s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001478s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001785s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002142s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000322s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000441s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000312s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000770s ]
