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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated MAPK6 Recombinant Rabbit mAb

#65580

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using MAPK6 antibody (Cat#65580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPK6 antibody (Cat#65580). MAPK6 expression in wild-type (WT) and MAPK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MAPK6 expression in HAP-1 cells using MAPK6 antibody (Cat#65580, 1:2,000). Green, isotype control; red, MAPK6.
Validation of MAPK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MAPK6 antibody (Cat#65580, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    MAPK6
  • Catalog：
    65580
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated MAPK6 Recombinant Rabbit mAb
Aliases MAPK6; Mitogen-Activated Protein Kinase 6; ERK3; HsT17250; P97MAPK; PRKM6; Extracellular Signal-Regulated Kinase 3; MAP Kinase 6; EC 2.7.11.24; P97-MAPK; MAPK 6; ERK-3; Extracellular Signal-Regulated Kinase, P97; Protein Kinase, Mitogen-Activated 5; Protein Kinase, Mitogen-Activated 6; MAP Kinase Isoform P97; EC 2.7.11
Background

Gene Name: MAPK6

NCBI Gene Entry: 5597

UniProt Entry: Q16659

Application Information

Molecular Weight: Predicted, 83 kDa, observed, 105 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4360

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human MAPK6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using MAPK6 antibody (Cat#65580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using MAPK6 antibody (Cat#65580). MAPK6 expression in wild-type (WT) and MAPK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of MAPK6 expression in HAP-1 cells using MAPK6 antibody (Cat#65580, 1:2,000). Green, isotype control; red, MAPK6.
  • Validation of MAPK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MAPK6 antibody (Cat#65580, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    MAPK6
  • Catalog：
    65580
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using MAPK6 antibody (Cat#65580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPK6 antibody (Cat#65580). MAPK6 expression in wild-type (WT) and MAPK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK6 antibody (Cat#65580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MAPK6 expression in HAP-1 cells using MAPK6 antibody (Cat#65580, 1:2,000). Green, isotype control; red, MAPK6.
Validation of MAPK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MAPK6 antibody (Cat#65580, 1:2,000) and analyzed using BD flow cytometer.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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All Rights Reserved.

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  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000555s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000824s ]
  3. SELECT * FROM `goods` WHERE `code` = '65580' LIMIT 1 [ RunTime:0.000762s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000632s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000505s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000563s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 8581 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000574s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000640s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 8581 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000412s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000603s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000397s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.059378s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7768,9652,3818,9132,9839,9611,5010,6006,9684,5961) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000783s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000651s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000242s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000449s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000240s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000287s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000384s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000245s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000328s ]
0.100679s