Western blotting analysis using Seh1L antibody (Cat#65601). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Seh1L antibody (Cat#65601, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Seh1L antibody (Cat#65601). Seh1L expression in wild-type (WT) and SEH1L shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Seh1L antibody (Cat#65601, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse testis tissue using seh1L antibody (Cat#65601, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HeLa cells using Seh1L antibody (Cat#65601, 1:1,000), Top panel: wild-type (WT); Bottom panal: Seh1L shRNA knockdown (KD). Nuclei were stained blue with DAPI; Seh1L was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Immunocytochemical staining of HAP-1 cells with Seh1L antibody (Cat#65601, 1:1,000) . Nuclei were stained blue with DAPI; Seh1L was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Seh1L expression in HAP-1 cells using Seh1L antibody (Cat#65601, 1:2,000). Green, isotype control; red, Seh1L.
Validation of Seh1L knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Seh1L antibody (Cat#65601, 1:2,000) and analyzed using BD flow cytometer.
