Western blotting analysis using serine/threonine kinase 33 antibody (Cat#65607). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with serine/threonine kinase 33 antibody (Cat#65607, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using serine/threonine kinase 33 antibody (Cat#65607). Serine/threonine kinase 33 expression in wild-type (WT) and serine/threonine kinase 33 (STK33) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with serine/threonine kinase 33 antibody (Cat#65607, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Serine/threonine kinase 33 expression in HT-1080 cells using Serine/threonine kinase 33 antibody (Cat#65607, 1:2,000). Green, isotype control; red, Serine/threonine kinase 33.
Validation of Serine/threonine kinase 33 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Serine/threonine kinase 33 antibody (Cat#65607, 1:2,000) and analyzed using BD flow cytometer.