Western blotting analysis using striatin 4 antibody (Cat#65608). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with striatin 4 antibody (Cat#65608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using striatin 4 antibody (Cat#65608). Striatin 4 expression in wild-type (WT) and striatin 4 (STRN4) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with striatin 4 antibody (Cat#65608, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Striatin 4 expression in HAP-1 cells using Striatin 4 antibody (Cat#65608, 1:2,000). Green, isotype control; red, Striatin 4.
Validation of Striatin 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Striatin 4 antibody (Cat#65608, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HAP-1 cells with Striatin 4 antibody(Cat#65608, 1:1,000) . Nuclei were stained blue with DAPI; Striatin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
