Western blotting analysis using Integrin beta 1 antibody (Cat#65731). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin beta 1 antibody (Cat#65731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Integrin beta 1 antibody (Cat#65731). Integrin beta 1 expression in wild type (WT) and Integrin beta 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Integrin beta 1 antibody (Cat#65731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Integrin beta 1 expression in HT-1080 cells using Integrin beta 1 antibody (Cat#65731, 1:2,000). Green, isotype control; red, Integrin beta 1.
Immunocytochemical staining of HT-1080 cells with Integrin beta 1 antibody (Cat#65731, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using integrin beta1 antibody (Cat#65731, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
