Western blotting analysis using TTLL12 antibody (Cat#65831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using TTLL12 antibody (Cat#65831). TTLL12 expression in wild-type (WT) and TTLL12 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TTLL12 antibody (Cat#65831, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TTLL12 expression in HepG2 cells using TTLL12 antibody (Cat#65831, 1:2,000). Green, isotype control; red, TTLL12.
Validation of TTLL12 knockdown using flow cytometry. Wild-type(WT, Blue) and TTLL12 knockdown(KD, Green) HeLa cells were stained with TTLL12 antibody(Cat#65831, 1:2,000) and analyzed using BD flow cytometer.
