Western blotting analysis using COX6C antibody (Cat#65867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COX6C antibody (Cat#65867, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using COX6C antibody (Cat#65867). COX6C expression in wild-type (WT) and COX6C shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with COX6C antibody (Cat#65867, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with COX6C antibody(Cat#65867, 1:1,000) . Nuclei were stained blue with DAPI; COX6C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using COX6C antibody (Cat#65867, 1:1,000), Top panel: wild-type (WT); Bottom panal: COX6C shRNA knockdown (KD). Nuclei were stained blue with DAPI; COX6C was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of COX6C expression in HAP-1 cells using COX6C antibody (Cat#65867, 1:2,000). Green, isotype control; red, COX6C.
Validation of COX6C knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with COX6C antibody (Cat#65867, 1:2,000) and analyzed using BD flow cytometer.
