Western blotting analysis using HSD17B4 antibody (Cat#65880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSD17B4 antibody (Cat#65880). HSD17B4 expression in wild-type (WT) and HSD17B4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSD17B4 antibody (Cat#65880, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using HSD17B4 antibody (Cat#65880, 1:1,000), Top panel: wild-type (WT); Bottom panal: HSD17B4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells with HSD17B4 antibody (Cat#65880, 1:1,000) . Nuclei were stained blue with DAPI; HSD17B4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of HSD17B4 expression in HepG2 cells using HSD17B4 antibody (Cat#65880,1:2,000). Green, isotype control; red, HSD17B4.
Validation of HSD17B4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HSD17B4 antibody (Cat#65880, 1:2,000) and analyzed using BD flow cytometer.
