Western blotting analysis using LRCH4 antibody (Cat#65902). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using LRCH4 antibody (Cat#65902). LRCH4 expression in wild-type (WT) and LRCH4 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LRCH4 antibody (Cat#65902, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of LRCH4 expression in HT-1080 cells using LRCH4 antibody (Cat#65902, 1:2,000). Green, isotype control; red, LRCH4.
Validation of LRCH4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with LRCH4 antibody (Cat#65902, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HT-1080 cells using LRCH4 antibody (Cat#65902, 1:1,000), Top panel: wild-type (WT); Bottom panal: LRCH4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LRCH4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
