Western blotting analysis using S100A16 antibody (Cat#6591). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S100A16 antibody (Cat#6591, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with S100A16 antibody (Cat#6591, 1:1,000). Nuclei were stained blue with DAPI; S100A16 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of S100A16 expression in HaCaT cells using S100A16 antibody (Cat#6591, 1:2,000). Green, isotype control; red, S100A16.