Western blotting analysis using STIP1 antibody (Cat#6602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STIP1 antibody (Cat#6602, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of STIP1 expression in HAP-1 cells using STIP1 antibody (Cat#6602, 1:2,000). Green, isotype control; red, STIP1.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human STIP1
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using STIP1 antibody (Cat#6602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STIP1 antibody (Cat#6602, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of STIP1 expression in HAP-1 cells using STIP1 antibody (Cat#6602, 1:2,000). Green, isotype control; red, STIP1.
Western blotting analysis using STIP1 antibody (Cat#6602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STIP1 antibody (Cat#6602, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of STIP1 expression in HAP-1 cells using STIP1 antibody (Cat#6602, 1:2,000). Green, isotype control; red, STIP1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000772s ]
SELECT * FROM `goods` WHERE `code` = '6602' LIMIT 1 [ RunTime:0.000504s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000398s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000349s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000377s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10358 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000342s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000466s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10358 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000268s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000365s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000254s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.057644s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5523,7936,6859,8032,163,3902,9044,10115,10530,5489) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000819s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000448s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001967s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000578s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000220s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002411s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000382s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000291s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000230s ]
