Western blotting analysis using DNPH1 antibody (Cat#6610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNPH1 antibody (Cat#6610, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNPH1 expression in Jurkat cells using DNPH1 antibody (Cat#6610, 1:2,000). Green, isotype control; red, DNPH1.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DNPH1
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using DNPH1 antibody (Cat#6610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNPH1 antibody (Cat#6610, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNPH1 expression in Jurkat cells using DNPH1 antibody (Cat#6610, 1:2,000). Green, isotype control; red, DNPH1.
Western blotting analysis using DNPH1 antibody (Cat#6610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNPH1 antibody (Cat#6610, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DNPH1 expression in Jurkat cells using DNPH1 antibody (Cat#6610, 1:2,000). Green, isotype control; red, DNPH1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000713s ]
SELECT * FROM `goods` WHERE `code` = '6610' LIMIT 1 [ RunTime:0.000619s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001102s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000686s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001292s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10363 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000572s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001462s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10363 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000870s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000860s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.001658s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.146526s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10099,8917,6593,8283,4753,7786,9645,10188,9564,4202) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003901s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.006254s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000722s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001433s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.004519s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000556s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000697s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.001759s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000542s ]
