Western blotting analysis using PLIN3 antibody (Cat#66108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PLIN3 antibody (Cat#66108, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PLIN3 antibody (Cat#66108). PLIN3 expression in wild-type (WT) and PLIN3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PLIN3 antibody (Cat#66108, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PLIN3 expression in HepG2 cells using PLIN3 antibody (Cat#66108, 1:2,000). Green, isotype control; red, PLIN3.