Western blotting analysis using FAM120A antibody (Cat#66131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FAM120A antibody (Cat#66131, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FAM120A antibody (Cat#66131). FAM120A expression in wild-type (WT) and FAM120A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FAM120A antibody (Cat#66131, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FAM120A expression in HepG2 cells using FAM120A antibody (Cat#66131, 1:2,000). Green, isotype control; red, FAM120A.
Validation of FAM120A knockdown using flow cytometry. Wild-type(WT, Blue) and FAM120A knockdown(KD, Green) HeLa cells were stained with FAM120A antibody(Cat#66131, 1:2,000) and analyzed using BD flow cytometer.