Western blotting analysis using WBP2 antibody (Cat#66139). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WBP2 antibody (Cat#66139, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using WBP2 antibody (Cat#66139). WBP2 expression in wild-type (WT) and WBP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with WBP2 antibody (Cat#66139, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of WBP2 expression in HepG2 cells using WBP2 antibody (Cat#66139,1:2,000). Green, isotype control; red, WBP2.
Validation of WBP2 knockdown using flow cytometry. Wild-type(WT, Blue) and WBP2 knockdown(KD, Green) HeLa cells were stained with WBP2 antibody(Cat#66139, 1:2,000) and analyzed using BD flow cytometer.
