Western blotting analysis using HK2 antibody (Cat#66191). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HK2 antibody (Cat#66191, 1:2,000) and HRP-conjugated goat mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HK2 antibody (Cat#66191). HK2 expression in wild-type (WT) and HK2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HK2 antibody (Cat#66191, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HK2 expression in HAP-1 cells using HK2 antibody (Cat#66191, 1:2,000). Green, isotype control; red, HK2.
Validation of HK2 knockdown using flow cytometry. Wild-type(WT, Blue) and HK2 knockdown(KD, Green) HeLa cells were stained with HK2 antibody(Cat#66191, 1:2,000) and analyzed using BD flow cytometer.
