Western blotting analysis using ST13 antibody (Cat#66226). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ST13 antibody (Cat#66226, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ST13 antibody (Cat#66226). ST13 expression in wild-type (WT) and ST13 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ST13 antibody (Cat#66226, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ST13 antibody (Cat#66226, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HAP1 cells with ST13 antibody (Cat#66226,1:1,000). Nuclei were stained blue with DAPI; ST13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of ST13 expression in HAP-1 cells using ST13 antibody (Cat#66226,1:2,000). Green, isotype control; red, ST13.
