Western blotting analysis using tumor protein p53 antibody (Cat#6624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tumor protein p53 antibody (Cat#6624, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with tumor protein p53 antibody (Cat#6624, 1:1,000). Nuclei were stained blue with DAPI; Tumor protein p53 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of tumor protein p53 expression in HeLa cells using tumor protein p53 antibody (Cat#6624, 1:2,000). Green, isotype control; red, tumor protein p53
