Western blotting analysis using cdk4 antibody (Cat#66244). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cdk4 antibody (Cat#66244,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using cdk4 antibody (Cat#66244). Cdk4 expression in wild type (WT) and cdk4 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cdk4 antibody (Cat#66244, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human cervical carcinoma using cdk4 antibody (Cat#66244, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with cdk4 antibody (Cat#66244, 1:1,000). Nuclei were stained blue with DAPI; Cdk4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of cdk4 expression in HepG2 cells using cdk4 antibody (Cat#66244,1:2,000). Green, isotype control; red, Cdk4.
Validation of cdk4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with cdk4 antibody (Cat#66244, 1:2,000) and analyzed using BD flow cytometer.
