Western blotting analysis using cathepsin B antibody (Cat#6625). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin B antibody (Cat#6625, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CTSB expression in HaCaT cells using CTSB antibody (Cat#6625, 1:2,000). Green, isotype control; red, CTSB.
Applications Tested: Western Blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human Cathepsin B
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using cathepsin B antibody (Cat#6625). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin B antibody (Cat#6625, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CTSB expression in HaCaT cells using CTSB antibody (Cat#6625, 1:2,000). Green, isotype control; red, CTSB.
Western blotting analysis using cathepsin B antibody (Cat#6625). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin B antibody (Cat#6625, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CTSB expression in HaCaT cells using CTSB antibody (Cat#6625, 1:2,000). Green, isotype control; red, CTSB.
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