Western blotting analysis using DLD antibody (Cat#66447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DLD antibody (Cat#66447, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DLD antibody (Cat#66447). DLD expression in wild-type (WT) and DLD shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DLD antibody (Cat#66447, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with DLD antibody (Cat#66447, 1:1,000) . Nuclei were stained blue with DAPI; DLD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Validation of DLD knockdown using flow cytometry. Wild-type(WT, Blue) and DLD knockdown(KD, Green) HeLa cells were stained with DLD antibody(Cat#66447, 1:2,000) and analyzed using BD flow cytometer.
