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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated Tubulin Beta 3 Class III Mouse mAb

#66488

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shRNA Knockdown Validated
Western blotting analysis using tubulin beta 3 class III antibody (Cat#66488). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tubulin beta 3 class III antibody (Cat#66488, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using tubulin beta 3 class III antibody (Cat#66488). TUBB3 expression in wild-type (WT) and tubulin beta 3 class III (TUBB3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with tubulin beta 3 class III antibody (Cat#66488, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with TUBB3 antibody (Cat#66488, 1:1,000). Nuclei were stained blue with DAPI; TUBB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    TUBB3
  • Catalog：
    66488
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Tubulin Beta 3 Class III Mouse mAb
Aliases TUBB3; Tubulin Beta 3 Class III; CFEOM3A; Beta-4; CFEOM3; Class III Beta-Tubulin; Tubulin Beta-3 Chain; Tubulin Beta-4 Chain; Tubulin Beta-III; Tubulin, Beta 3; FEOM3; TUBB4; Fibrosis Of Extraocular Muscles, Congenital, 3; CDCBM1; CDCBM
Background

Gene Name: TUBB3

NCBI Gene Entry: 10381

UniProt Entry: Q13509

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9550

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human TUBB3
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using tubulin beta 3 class III antibody (Cat#66488). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tubulin beta 3 class III antibody (Cat#66488, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using tubulin beta 3 class III antibody (Cat#66488). TUBB3 expression in wild-type (WT) and tubulin beta 3 class III (TUBB3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with tubulin beta 3 class III antibody (Cat#66488, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with TUBB3 antibody (Cat#66488, 1:1,000). Nuclei were stained blue with DAPI; TUBB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    TUBB3
  • Catalog：
    66488
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using tubulin beta 3 class III antibody (Cat#66488). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tubulin beta 3 class III antibody (Cat#66488, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using tubulin beta 3 class III antibody (Cat#66488). TUBB3 expression in wild-type (WT) and tubulin beta 3 class III (TUBB3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with tubulin beta 3 class III antibody (Cat#66488, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with TUBB3 antibody (Cat#66488, 1:1,000). Nuclei were stained blue with DAPI; TUBB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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    8:30 AM - 5:30 PM EST Mon-Fri

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  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000482s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001036s ]
  3. SELECT * FROM `goods` WHERE `code` = '66488' LIMIT 1 [ RunTime:0.000660s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000454s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000331s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000414s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10374 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000408s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000618s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10374 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000324s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000402s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000276s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.067845s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4265,5524,6158,4259,4989,8157,5913,9672,5461,6380) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000877s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000484s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000219s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000447s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000216s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000305s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000376s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000246s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000197s ]
0.107903s