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  • Primary Antibody
  • Secondary Antibodies
  • Lentivirus
  • Cell  Lines
  • Lysates
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Home>Product>Primary Antibody

KD-Validated ABCB1 Mouse mAb

#66490

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shRNA Knockdown Validated
Western blotting analysis using ABCB1 antibody (Cat#66490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ABCB1 antibody (Cat#66490). ABCB1 expression in wild-type (WT) and ABCB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ABCB1
  • Catalog：
    66490
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ABCB1 Mouse mAb
Aliases ABCB1; ATP Binding Cassette Subfamily B Member 1; PGY1; Multidrug Resistance Protein 1; CD243; GP170; ABC20; P-170; P-Gp; MDR1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; ATP-Dependent Translocase ABCB1; Phospholipid Transporter ABCB1; Colchicin Sensitivity; P-Glycoprotein 1; CLCS; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Resistance; P-Glycoprotein; CD243 Antigen; EC 3.6.3.44; EC 7.6.2.2; EC 7.6.2.1; EC 3.6.3; ENPAT
Background

Gene Name: ABCB1

NCBI Gene Entry: 5243

UniProt Entry: P08183

Application Information

Molecular Weight: Predicted, 141 kDa; observed, 141-250 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9560

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human ABCB1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using ABCB1 antibody (Cat#66490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ABCB1 antibody (Cat#66490). ABCB1 expression in wild-type (WT) and ABCB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ABCB1
  • Catalog：
    66490
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ABCB1 antibody (Cat#66490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ABCB1 antibody (Cat#66490). ABCB1 expression in wild-type (WT) and ABCB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000395s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000777s ]
  3. SELECT * FROM `goods` WHERE `code` = '66490' LIMIT 1 [ RunTime:0.000501s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000470s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000383s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000420s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10376 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000328s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000953s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10376 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000273s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000412s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000259s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.058818s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10545,6267,10045,4886,10311,9207,9084,10057,6739,5620) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000876s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000444s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000197s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001788s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000228s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000344s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.001146s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.002783s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000246s ]
0.107179s