Western blotting analysis using spermine synthase antibody (Cat#66587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with spermine synthase antibody (Cat#66587, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using spermine synthase antibody (Cat#66587). Spermine synthase expression in wild-type (WT) and spermine synthase (SMS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with spermine synthase antibody (Cat#66587, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using spermine synthase antibody (Cat#66587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with spermine synthase antibody (Cat#66587, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using spermine synthase antibody (Cat#66587). Spermine synthase expression in wild-type (WT) and spermine synthase (SMS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with spermine synthase antibody (Cat#66587, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using spermine synthase antibody (Cat#66587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with spermine synthase antibody (Cat#66587, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using spermine synthase antibody (Cat#66587). Spermine synthase expression in wild-type (WT) and spermine synthase (SMS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with spermine synthase antibody (Cat#66587, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000792s ]
SELECT * FROM `goods` WHERE `code` = '66587' LIMIT 1 [ RunTime:0.000498s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000452s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000326s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000439s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10380 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000333s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000422s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10380 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000294s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000377s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000248s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.086351s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4225,5297,6266,6581,9440,6444,7900,4308,4382,6712) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003049s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000458s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000206s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000509s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000204s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000295s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000395s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000239s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000199s ]
