Western blotting analysis using BAG2 antibody (Cat#6690). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAG2 antibody (Cat#6690, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with BAG2 antibody (Cat#6690, 1:1,000). Nuclei were stained blue with DAPI; BAG2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Flow cytometric analysis of BAG2 expression in HepG2 cells using BAG2 antibody (Cat#6690, 1:2,000). Green, isotype control; red, BAG2.