Western blotting analysis using GSK3 alpha/beta antibody (Cat#67431). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GSK3 alpha/beta antibody (Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using GSK3 alpha/beta antibody (Cat#67431). GSK3 alpha/beta expression in wildtype (WT) and GSK3 alpha/beta shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GSK3 alpha/beta antibody(Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201,1:20,000)respectively. lmage was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of GSK3 alpha/beta expression in HepG2 cells using GSK3 alpha/beta antibody (Cat#67431, 1:2,000). Green, isotype control; red, GSK3 alpha/beta.
Immunocytochemical staining of HepG2 cells with GSK3 alpha/beta antibody (Cat#67431, 1:1,000). Nuclei were stained blue with DAPI; GSK3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
