Western blotting analysis using DLAT antibody (Cat#6746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DLAT antibody (Cat#6746, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DLAT expression in HAP-1 cells using DLAT antibody (Cat#6746, 1:2,000). Green, isotype control; red, DLAT.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DLAT
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:4,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using DLAT antibody (Cat#6746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DLAT antibody (Cat#6746, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DLAT expression in HAP-1 cells using DLAT antibody (Cat#6746, 1:2,000). Green, isotype control; red, DLAT.
Western blotting analysis using DLAT antibody (Cat#6746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DLAT antibody (Cat#6746, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DLAT expression in HAP-1 cells using DLAT antibody (Cat#6746, 1:2,000). Green, isotype control; red, DLAT.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000789s ]
SELECT * FROM `goods` WHERE `code` = '6746' LIMIT 1 [ RunTime:0.003379s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001996s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000476s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000451s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10445 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001478s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002406s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10445 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001700s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000365s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000355s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.079963s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8399,9959,3987,8344,8927,188,8761,8832,8435,7271) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.005275s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000527s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.004314s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000556s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000243s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002196s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000421s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000267s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000198s ]
