Western blotting analysis using VHL antibody (Cat#6774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VHL antibody (Cat#6774, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
VHL; Von Hippel-Lindau Tumor Suppressor; VHL1; Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase; Von Hippel-Lindau Disease Tumor Suppressor; Protein G7; PVHL; Von Hippel-Lindau Syndrome; Elongin Binding Protein; HRCA1; RCA1
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using VHL antibody (Cat#6774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VHL antibody (Cat#6774, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using VHL antibody (Cat#6774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VHL antibody (Cat#6774, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000663s ]
SELECT * FROM `goods` WHERE `code` = '6774' LIMIT 1 [ RunTime:0.004332s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000418s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002287s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.003166s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 10472 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000851s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002207s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 10472 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002508s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000430s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000441s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.174155s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6410,6473,9594,7247,7297,4592,9105,6135,9120,9263) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004813s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.006100s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000271s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.003709s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002054s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000322s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.004133s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.002786s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001177s ]
