Western blotting analysis using ACSS2 antibody (Cat#69177). Total cell lysates (45 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1: 20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ACSS2, acyl-CoA synthetase short chain family member 2.
Western blotting analysis using ACSS2 antibody (Cat#69177). ACSS2 expression in wild type (WT) and ACSS2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ACSS2 expression in HepG2 cells using ACSS2 antibody (Cat#69177, 1:2,000). Green, isotype control; red, ACSS2.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human ACSS2
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using ACSS2 antibody (Cat#69177). Total cell lysates (45 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1: 20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ACSS2, acyl-CoA synthetase short chain family member 2.
Western blotting analysis using ACSS2 antibody (Cat#69177). ACSS2 expression in wild type (WT) and ACSS2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ACSS2 expression in HepG2 cells using ACSS2 antibody (Cat#69177, 1:2,000). Green, isotype control; red, ACSS2.
Western blotting analysis using ACSS2 antibody (Cat#69177). Total cell lysates (45 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1: 20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ACSS2, acyl-CoA synthetase short chain family member 2.
Western blotting analysis using ACSS2 antibody (Cat#69177). ACSS2 expression in wild type (WT) and ACSS2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ACSS2 expression in HepG2 cells using ACSS2 antibody (Cat#69177, 1:2,000). Green, isotype control; red, ACSS2.
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