Western blotting analysis using cyclin dependent kinase inhibitor 1B antibody (Cat#69245). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cyclin dependent kinase inhibitor 1B antibody (Cat#69245, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using cyclin dependent kinase inhibitor 1B antibody (Cat#69245). Cyclin dependent kinase inhibitor 1B expression in wild type (WT) and cyclin dependent kinase inhibitor 1B shRNA knockdown (KD) HAP-1 cells with 30μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with cyclin dependent kinase inhibitor 1B antibody (Cat#69245, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using cyclin dependent kinase inhibitor 1B antibody (Cat#69245, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using cyclin dependent kinase inhibitor 1B antibody (Cat#69245, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with cyclin dependent kinase inhibitor 1B antibody (Cat#69245, 1:1,000). Nuclei were stained blue with DAPI; cyclin dependent kinase inhibitor 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of cyclin dependent kinase inhibitor 1B expression in HAP-1 cells using cyclin dependent kinase inhibitor 1B antibody (Cat#69245, 1:2,000). Green, isotype control; red, Cyclin dependent kinase inhibitor 1B.
Validation of cyclin dependent kinase inhibitor 1B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with cyclin dependent kinase inhibitor 1B antibody (Cat#69245, 1:2,000) and analyzed using BD flow cytometer.
