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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated Phospho-PKR (T451) Recombinant Rabbit mAb

#69322

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Phospho-PKR (T451) antibody (Cat#69322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PKR (T451) antibody (Cat#69322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Phospho-PKR (T451) antibody (Cat#69322). Phospho-PKR (T451) expression in wild type (WT) and phospho-PKR (T451) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospho-PKR (T451) antibody (Cat#69322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-PKR (T451) expression in HT-1080 cells using Phospho-PKR (T451) antibody (Cat#69322, 1:2,000). Green, isotype control; red, Phospho-PKR (T451).
Validation of PKR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-PKR (T451) antibody (Cat#69322, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HT-1080 cells with Phospho-PKR (T451)  antibody (Cat#69322, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PKR (T451)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    EIF2AK2
  • Catalog：
    69322
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Phospho-PKR (T451) Recombinant Rabbit mAb
Aliases eIF-2A protein kinase 2; Protein kinase RNA-activated; PKR; p68 kinase; P1/eIF-2A protein kinase
Background

Gene Name: EIF2AK2

NCBI Gene Entry: 5610

UniProt Entry: P19525

Application Information

Molecular Weight: Predicted, 62 kDa, observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1710

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Phospho-PKR (T451)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-PKR (T451) antibody (Cat#69322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PKR (T451) antibody (Cat#69322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Phospho-PKR (T451) antibody (Cat#69322). Phospho-PKR (T451) expression in wild type (WT) and phospho-PKR (T451) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospho-PKR (T451) antibody (Cat#69322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-PKR (T451) expression in HT-1080 cells using Phospho-PKR (T451) antibody (Cat#69322, 1:2,000). Green, isotype control; red, Phospho-PKR (T451).
  • Validation of PKR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-PKR (T451) antibody (Cat#69322, 1:2,000) and analyzed using CytoFLEX.
  • Immunocytochemical staining of HT-1080 cells with Phospho-PKR (T451)  antibody (Cat#69322, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PKR (T451)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    EIF2AK2
  • Catalog：
    69322
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-PKR (T451) antibody (Cat#69322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PKR (T451) antibody (Cat#69322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Phospho-PKR (T451) antibody (Cat#69322). Phospho-PKR (T451) expression in wild type (WT) and phospho-PKR (T451) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospho-PKR (T451) antibody (Cat#69322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-PKR (T451) expression in HT-1080 cells using Phospho-PKR (T451) antibody (Cat#69322, 1:2,000). Green, isotype control; red, Phospho-PKR (T451).
Validation of PKR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-PKR (T451) antibody (Cat#69322, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HT-1080 cells with Phospho-PKR (T451)  antibody (Cat#69322, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PKR (T451)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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    sales@genuinbiotech.com

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  135. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  136. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  137. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  138. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  139. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  140. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/Raw.php ( 1.22 KB )
  141. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/View.php ( 1.70 KB )
  142. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/View.php ( 4.39 KB )
  143. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/view/driver/Php.php ( 6.44 KB )
  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000623s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000914s ]
  3. SELECT * FROM `goods` WHERE `code` = '69322' LIMIT 1 [ RunTime:0.000552s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000483s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000404s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000448s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 8723 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000523s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000690s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 8723 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000408s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000471s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000351s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054203s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6496,6107,10051,9515,8784,8167,4643,10201,10283,5520) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000811s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000575s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000792s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000658s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000364s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000472s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000536s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000360s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000309s ]
0.109851s