Western blotting analysis using BCL2 like 11 antibody (Cat#71146). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 like 11 antibody (Cat#71146, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using BCL2 like 11 antibody (Cat#71146). BCL2 like 11 expression in wild type (WT) and BCL2 like 11 (BCL2L11) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with BCL2 like 11 antibody (Cat#71146, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of BCL2 like 11 expression in HepG2 cells using BCL2 like 11 antibody (Cat# 71146, 1:2,000). Green, isotype control; red, BCL2 like 11.
Immunocytochemical staining of HepG2 cells with BCL2 like 11 antibody (Cat#71146, 1:1,000). Nuclei were stained blue with DAPI; BCL2 like 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
