Western blotting analysis using CD274 antibody (Cat#71191). CD274 expression in wild type (WT) and CD274 knockout (KO) HSHC cells with 50 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD274 antibody (Cat#71191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CD274 antibody (Cat#71191). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD274 antibody (Cat#71191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using CD274 antibody (Cat#71191, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen
A synthesized peptide derived from human PD-L1 (CD274)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note
This product is for research use only.
Data
Western blotting analysis using CD274 antibody (Cat#71191). CD274 expression in wild type (WT) and CD274 knockout (KO) HSHC cells with 50 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD274 antibody (Cat#71191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CD274 antibody (Cat#71191). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD274 antibody (Cat#71191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using CD274 antibody (Cat#71191, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using CD274 antibody (Cat#71191). CD274 expression in wild type (WT) and CD274 knockout (KO) HSHC cells with 50 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CD274 antibody (Cat#71191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CD274 antibody (Cat#71191). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD274 antibody (Cat#71191, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using CD274 antibody (Cat#71191, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000778s ]
SELECT * FROM `goods` WHERE `code` = '71191' LIMIT 1 [ RunTime:0.000490s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000426s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000363s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000376s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8808 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000334s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000490s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8808 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000319s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000504s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000358s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055345s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8434,5335,9395,3770,4397,6476,4362,6985,8921,7967) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000836s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000435s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000217s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000507s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000250s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000313s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000380s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000246s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000221s ]
