Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-MSK1 (S376) antibody (Cat#71200). Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human melanoma using phospho-MSK1 (S376) antibody (Cat#71200, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of H9c2 cells with Phospho-MSK1 (S376) antibody (Cat#71200, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using Phospho-MSK1 (S376) antibody (Cat#71200, 1:2,000). Green, isotype control; red, Phospho-MSK1 (S376).
