Western blotting analysis using apolipoprotein E antibody (Cat#71212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using apolipoprotein E antibody (Cat#71212). Apolipoprotein E expression in wild type (WT) and apolipoprotein E (APOE) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using apolipoprotein E antibody (Cat#71212). Apolipoprotein E expression in wild type (WT) and apolipoprotein E (APOE) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with apolipoprotein E antibody (Cat#71212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Apolipoprotein E antibody (Cat#71212, 1:1,000). Nuclei were stained blue with DAPI; Apolipoprotein E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
