Western blotting analysis using enolase 2 antibody (Cat#H1112). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with enolase 2 antibody (Cat#H1112, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using enolase 2 antibody (Cat#H1112). Enolase 2 expression in wild type (WT) and enolase 2 (ENO2) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with enolase 2 antibody (Cat#H1112, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows positive staining in paraffin-embedded human pancreas islet. NSE antibody (Cat#H1112) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. NSE antibody (Cat#H1112) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human cerebral cortex. NSE antibody (Cat#H1112) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
