Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in A549 cells using Erb-b2 receptor tyrosine kinase 2 antibody (Cat#H1113, 1:2,000). Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. ErbB2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. ErbB2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human breast. ErbB2 antibody (Cat#H1113) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
