Western blotting analysis using forkhead box P1 antibody (Cat#H1118). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with forkhead box P1 antibody (Cat#H1118, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using forkhead box P1 antibody (Cat#H1118). Forkhead box P1 expression in wild type (WT) and forkhead box P1 (FOXP1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with forkhead box P1 antibody (Cat#H1118, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Forkhead box P1 expression in HepG2 cells using Forkhead box P1 antibody (Cat#H1118, 1:2,000). Green, isotype control; red, Forkhead box P1.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast. FOXP1 antibody (Cat#H1118) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. FOXP1 antibody (Cat#H1118) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
