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  • Primary Antibody
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Home>Product>Primary Antibody

KO-Validated PTEN Recombinant Rabbit mAb

#H1128

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Western blotting analysis using PTEN antibody (Cat#H1128). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTEN antibody (Cat#H1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PTEN expression in Raji cells using PTEN antibody (Cat#H1128, 1:2,000). Green, isotype control; red, PTEN.
Immunocytochemical staining shows positive staining in paraffin-embedded human pancreas. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human cervical carcinoma. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human ovarian carcinoma. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse cerebral cortex. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded rat cerebral cortex. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Western blotting analysis using PTEN antibody (Cat#H1128). PTEN expression in wild type (WT) and PTEN knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PTEN antibody (Cat#H1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    PTEN
  • Catalog：
    H1128
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KO-Validated PTEN Recombinant Rabbit mAb
Aliases Phosphatase And Tensin Homolog; MMAC1; TEP1; Mutated In Multiple Advanced Cancers 1; PTEN1; Phosphatidylinositol 3,4,5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase PTEN; MHAM; BZS; Phosphatidylinositol-3,4,5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase PTEN; MMAC1 Phosphatase And Tensin Homolog Deleted On Chromosome 10; Mitochondrial Phosphatase And Tensin Protein Alpha; Phosphatase And Tensin-Like Protein; PTEN Variant PTEN-R234Afs*11; Protein Tyrosine Phosphatase; PTEN Variant PTEN-K267Rfs*9; PTEN Variant PTEN-L247*; Mitochondrial PTENalpha; PTENepsilon; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67; 10q23del; PTENbeta; CWS1; GLM2; DEC;
Background

Gene Name: PTEN

NCBI Gene Entry: 5728

UniProt Entry: P60484

Application Information

Molecular Weight: Predicted, 47 kDa, Observed, 56 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-R069

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P), flow cytometry (FCM)
Immunogen Recombinant protein of human PTEN
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PTEN antibody (Cat#H1128). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTEN antibody (Cat#H1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PTEN expression in Raji cells using PTEN antibody (Cat#H1128, 1:2,000). Green, isotype control; red, PTEN.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human pancreas. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human cervical carcinoma. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human colon. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human ovarian carcinoma. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded mouse cerebral cortex. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded rat cerebral cortex. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Western blotting analysis using PTEN antibody (Cat#H1128). PTEN expression in wild type (WT) and PTEN knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PTEN antibody (Cat#H1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    PTEN
  • Catalog：
    H1128
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PTEN antibody (Cat#H1128). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTEN antibody (Cat#H1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PTEN expression in Raji cells using PTEN antibody (Cat#H1128, 1:2,000). Green, isotype control; red, PTEN.
Immunocytochemical staining shows positive staining in paraffin-embedded human pancreas. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human cervical carcinoma. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human ovarian carcinoma. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse cerebral cortex. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded rat cerebral cortex. PTEN antibody (Cat#H1128) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Western blotting analysis using PTEN antibody (Cat#H1128). PTEN expression in wild type (WT) and PTEN knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PTEN antibody (Cat#H1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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  114. /www/wwwroot/sbywcs.dsger.com/app/web/controller/Product.php ( 24.51 KB )
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  120. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/ConnectionInterface.php ( 4.57 KB )
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  1. CONNECT:[ UseTime:0.000584s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001046s ]
  3. SELECT * FROM `goods` WHERE `code` = 'H1128' LIMIT 1 [ RunTime:0.000652s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000628s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000491s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000523s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 18 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000753s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000551s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 18 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000424s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000478s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000353s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.059939s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4115,345,4996,7931,8293,10458,8346,5460,6034,7124) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000950s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000615s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000283s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000530s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000306s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000293s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000412s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000280s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000328s ]
0.111242s