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  • Primary Antibody
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Home>Product>Primary Antibody

POU5F1 Recombinant Rabbit mAb

#H1129

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Western blotting analysis using POU class 5 homeobox 1 antibody (Cat#H1129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU class 5 homeobox 1 antibody (Cat#H1129, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining shows positive staining in paraffin-embedded human seminoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human hepatocellular carcinoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human cervical carcinoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human kidney. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human testis. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    POU5F1
  • Catalog：
    H1129
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name POU5F1 Recombinant Rabbit mAb
Aliases POU5F1; POU Class 5 Homeobox 1; OCT3; Oct3/4; OCT-4; Oct4; OTF3; POU Domain, Class 5, Transcription Factor 1; Octamer-Binding Transcription Factor 3; Octamer-Binding Protein 3; Octamer-Binding Protein 4; MGC22487; OTF-3; Oct-3; POU-Type Homeodomain-Containing DNA-Binding Protein; POU Domain Class 5, Transcription Factor 1; POU Domain Transcription Factor OCT4; Oct-4; OTF4; OCT4
Background

Gene Name: POU5F1

NCBI Gene Entry: 5460

UniProt Entry: Q01860

Application Information

Molecular Weight: Predicted, 39 kDa, Observed, 40 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-165-76

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human OCT3/4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using POU class 5 homeobox 1 antibody (Cat#H1129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU class 5 homeobox 1 antibody (Cat#H1129, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining shows positive staining in paraffin-embedded human seminoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human hepatocellular carcinoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human cervical carcinoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human kidney. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human testis. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    POU5F1
  • Catalog：
    H1129
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using POU class 5 homeobox 1 antibody (Cat#H1129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU class 5 homeobox 1 antibody (Cat#H1129, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining shows positive staining in paraffin-embedded human seminoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human hepatocellular carcinoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human cervical carcinoma. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human kidney. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human testis. OCT3/4 antibody (Cat#H1129) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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All Rights Reserved.

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  129. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/ParamsBind.php ( 3.66 KB )
  130. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/ResultOperation.php ( 7.01 KB )
  131. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/WhereQuery.php ( 19.37 KB )
  132. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/JoinAndViewQuery.php ( 7.11 KB )
  133. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/TableFieldInfo.php ( 2.63 KB )
  134. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/Transaction.php ( 2.77 KB )
  135. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  136. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  137. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  138. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  139. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  140. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/Raw.php ( 1.22 KB )
  141. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/View.php ( 1.70 KB )
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  143. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/view/driver/Php.php ( 6.44 KB )
  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000518s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000729s ]
  3. SELECT * FROM `goods` WHERE `code` = 'H1129' LIMIT 1 [ RunTime:0.000610s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000533s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000463s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000443s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 19 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000517s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000497s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 19 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000282s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000418s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000240s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053151s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5357,5659,8492,326,5478,10401,4383,9049,7631,5768) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000863s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000448s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000289s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000459s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000247s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000299s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000352s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000259s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000241s ]
0.093324s