Western blotting analysis using chorionic gonadotropin subunit beta 3 antibody (Cat#H1151). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chorionic gonadotropin subunit beta 3 antibody (Cat#H1151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Chorionic gonadotropin subunit beta 3 expression in A549 cells using Chorionic gonadotropin subunit beta 3 antibody (Cat#H1151, 1:2,000). Green, isotype control; red, Chorionic gonadotropin subunit beta 3.
Immunocytochemical staining shows positive staining in paraffin-embedded human placenta. CGB/HCG-β antibody (Cat#H1151) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human cerebral cortex. CGB/HCG-β antibody (Cat#H1151) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human tonsil. CGB/HCG-β antibody (Cat#H1151) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human liver. CGB/HCG-β antibody (Cat#H1151) was used at 1:1,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.