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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated Phospho-GSK-3 Beta (S9) Rabbit mAb

#R0006

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Knockdown Validated
Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Phospho-GSK-3β (Ser9) expression in wild-type (WT) and GSK3B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-GSK-3β (Ser9) expression in HepG2 cells using anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:2,000). Green, isotype control; red, Phospho-GSK-3β (Ser9).
Immunocytochemical staining of HepG2 cells with anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-GSK-3β (Ser9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    GSK3B
  • Catalog：
    R0006
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Phospho-GSK-3 Beta (S9) Rabbit mAb
Aliases GSK3B; Glycogen Synthase Kinase 3 Beta; Serine/Threonine-Protein Kinase GSK3B; Glycogen Synthase Kinase-3 Beta; EC 2.7.11.26; GSK-3 Beta; EC 2.7.11.1; EC 2.7.11
Background

Gene Name: GSK3B

NCBI Gene Entry: 2932

UniProt Entry: P49841

Application Information

Molecular Weight: Predicted, 47 kDa, Observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-748-20

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-GSK-3 Beta (S9)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Phospho-GSK-3β (Ser9) expression in wild-type (WT) and GSK3B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Phospho-GSK-3β (Ser9) expression in HepG2 cells using anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:2,000). Green, isotype control; red, Phospho-GSK-3β (Ser9).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-GSK-3β (Ser9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    GSK3B
  • Catalog：
    R0006
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006). Phospho-GSK-3β (Ser9) expression in wild-type (WT) and GSK3B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-GSK-3β (Ser9) expression in HepG2 cells using anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:2,000). Green, isotype control; red, Phospho-GSK-3β (Ser9).
Immunocytochemical staining of HepG2 cells with anti-Phospho-GSK-3β (Ser9) antibody (Cat#R0006, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-GSK-3β (Ser9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  136. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  137. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  138. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  139. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  140. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/Raw.php ( 1.22 KB )
  141. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/View.php ( 1.70 KB )
  142. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/View.php ( 4.39 KB )
  143. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/view/driver/Php.php ( 6.44 KB )
  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
  148. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/mobile.php ( 4.08 KB )
  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000460s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000811s ]
  3. SELECT * FROM `goods` WHERE `code` = 'R0006' LIMIT 1 [ RunTime:0.000473s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000445s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000458s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000490s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 337 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000432s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000502s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 337 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000266s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000412s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000246s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.074932s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6420,4608,7109,4721,6712,10559,4427,7739,9256,4895) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001181s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000564s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000205s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000486s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000263s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000373s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000441s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000247s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000200s ]
0.113760s